This reagent kit utilizes a silicon-based material that can efficiently and specifically bind to DNA, along with a unique buffer system. It recovers DNA fragments from TAE or TBE agarose gels, while eliminating impurities such as proteins, other organic compounds, inorganic salt ions, and oligonucleotide primers. It can recover fragments ranging in size from 100 bp to 30 kb, with a recovery rate of over 80%. Each centrifuge absorption column can absorb up to 20μg of DNA. The DNA recovered using this reagent kit can be used for various standard operations, including enzyme digestion,PCR, sequencing, library screening, ligation, and transformation experiments.